The painting is located in the Great Hall of the Upper Scuola Grande di San Rocco. The large proportions of the canvas are more accentuated by the canteen arranged diagonally towards the depth, which gives the illusion of an even larger space. This Last Supper is the moment when Christ, located at the end of the table, approached the host at the mouth of St. Peter depicting the moment of the Eucharist. St. John soon disappears almost visible lying on the table, sadly for the crippling burden of knowing the imminent betrayal and the identity of the traitor. Judah sat at the right hand of Christ, he leads to belt the bag with the money, noticed by one of the apostles who stands up leaning on the table in front of him.
In the foreground the monumental figures of two beggars lie. They have just received alms of bread and wine, to remember both the charitable purposes of the School is the Eucharistic miracle in progress, highlighted by the excitement that involves the apostles and the gesture Christ.
The light highlights the dramatic moments of the episode, in a continuous alternation of light and shade, highlighting the colors of the clothes of the protagonists; the light source is doubled, the light enters from the first floor is the door at the bottom right corner of the room, illuminating the interior of the kitchen with the servants busy in their duties.